Constitutive activation of the PDR16 promoter in a Candida albicans azole-resistant clinical isolate overexpressing CDR1 and CDR2

Antimicrob Agents Chemother. 2004 Jul;48(7):2700-3. doi: 10.1128/AAC.48.7.2700-2703.2004.

Abstract

Candida albicans azole-resistant clinical isolates overexpressing the CDR1 and CDR2 genes (multidrug transporters) also overexpress the PDR16 gene (phosphatidylinositol transfer protein). We show here that the PDR16 promoter displays higher transcriptional activity following integration in an azole-resistant isolate than in the matched azole-susceptible one. Thus, the upregulation of PDR16 in the resistant strain results from a mutation acting in trans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters / biosynthesis*
  • ATP-Binding Cassette Transporters / genetics
  • Antifungal Agents / pharmacology*
  • Azoles / pharmacology*
  • Base Sequence
  • Candida albicans / drug effects*
  • Carrier Proteins / genetics*
  • DNA Primers / genetics
  • DNA, Fungal / biosynthesis
  • DNA, Fungal / genetics
  • Databases, Genetic
  • Flow Cytometry
  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / genetics
  • Membrane Proteins / genetics*
  • Membrane Transport Proteins / biosynthesis*
  • Membrane Transport Proteins / genetics
  • Molecular Sequence Data
  • Mutation
  • Phospholipid Transfer Proteins
  • Promoter Regions, Genetic / genetics*
  • Up-Regulation / genetics

Substances

  • ATP-Binding Cassette Transporters
  • Antifungal Agents
  • Azoles
  • CDR1 protein, Candida albicans
  • Carrier Proteins
  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • Phospholipid Transfer Proteins