In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay

Abdalla O A Ahmed; Wendy W J van de Sande; Wim van Vianen; Alex van Belkum; Ahmed H Fahal; Henri A Verbrugh; Irma A J M Bakker-Woudenberg

doi:10.1128/AAC.48.7.2742-2746.2004

In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay

Antimicrob Agents Chemother. 2004 Jul;48(7):2742-6. doi: 10.1128/AAC.48.7.2742-2746.2004.

Authors

Abdalla O A Ahmed¹, Wendy W J van de Sande, Wim van Vianen, Alex van Belkum, Ahmed H Fahal, Henri A Verbrugh, Irma A J M Bakker-Woudenberg

Affiliation

¹ Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

Abstract

Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC(50)]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC(50) of 0.5 to 1.0 mg/liter).

MeSH terms

Amphotericin B / pharmacology*
Antifungal Agents / pharmacology*
Colorimetry
Itraconazole / pharmacology*
Madurella / drug effects*
Microbial Sensitivity Tests
Mycetoma / microbiology
Nephelometry and Turbidimetry
Reproducibility of Results
Tetrazolium Salts*

Substances

Antifungal Agents
Tetrazolium Salts
2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide
Itraconazole
Amphotericin B