Identification of a putative voltage-gated Ca2+ -permeable channel (OsTPC1) involved in Ca2+ influx and regulation of growth and development in rice

Plant Cell Physiol. 2004 Jun;45(6):693-702. doi: 10.1093/pcp/pch082.

Abstract

Cytosolic free Ca2+ serves as an important second messenger participating in signal transduction of various environmental stresses. However, molecular bases for the plasma membrane Ca2+ influx and its regulation remain largely unknown. We here identified a gene (OsTPC1) encoding a putative voltage-gated Ca2+ channel from rice, ubiquitously expressed in mature leaves, shoots and roots as well as in cultured cells. OsTPC1 rescued the Ca2+ uptake activity and growth rate of a yeast mutant cch1. To elucidate its physiological roles, we generated transgenic rice plants and cultured cells overexpressing OsTPC1 mRNA. Furthermore, a retrotransposon (Tos17) insertional knockout mutant of OsTPC1 was isolated. OsTPC1-overexpressing cells showed hypersensitivity to excess Ca2+ but higher growth rate under Ca2+ limitation, while growth of the OsTPC1-knockout cultured cells was less sensitive to extracellular free Ca2+ concentration, suggesting that OsTPC1 has Ca2+ transport activity across the plasma membrane. OsTPC1-overexpressing plants showed reduced growth and abnormal greening of roots. Growth of Ostpc1 seedlings was comparable to the control on agar plates, while significantly reduced in adult plants. These results suggest that OsTPC1 functions as a Ca2+ -permeable channel involved in the regulation of growth and development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Calcium Channels / genetics
  • Calcium Channels / isolation & purification
  • Calcium Channels / metabolism*
  • Calcium Signaling / genetics
  • Cell Membrane / genetics
  • Cell Membrane / metabolism*
  • Cells, Cultured
  • Gene Expression Regulation, Developmental / genetics
  • Gene Expression Regulation, Plant / genetics
  • Molecular Sequence Data
  • Mutation / genetics
  • Oryza / genetics
  • Oryza / growth & development*
  • Oryza / metabolism*
  • Plant Leaves / genetics
  • Plant Leaves / growth & development
  • Plant Leaves / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification
  • Plant Proteins / metabolism*
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • RNA, Messenger / metabolism
  • Seedlings / genetics
  • Seedlings / growth & development
  • Seedlings / metabolism
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • Calcium Channels
  • Plant Proteins
  • RNA, Messenger
  • Calcium

Associated data

  • GENBANK/AB071014