Acidic oligosaccharide sugar chain, a marine-derived acidic oligosaccharide, inhibits the cytotoxicity and aggregation of amyloid beta protein

J Pharmacol Sci. 2004 Jun;95(2):248-55. doi: 10.1254/jphs.fpj04004x.


In this paper, we investigated interactions of the acidic oligosaccharide sugar chain (AOSC), derived from brown algae Echlonia kurome OKAM, with amyloid beta protein (Abeta). We observed that AOSC inhibited the toxicity induced by Abeta in both primarily cortical cells and the SH-SY5Y cell line. We also observed that AOSC inhibited the apoptosis induced by Abeta in SH-SY5Y by reducing the elevated level of intracellular calcium concentration ([Ca(2+)](i)) and suppressing the generation of reactive oxygen species. Surface plasmon resonance analysis demonstrated that AOSC had affinity for both freshly-dissolved Abeta and 48-h incubated Abeta. Furthermore, AOSC blocked the fibril formation of Abeta, which may be responsible for its anti-cytotoxic effects. Thus, our results indicate that AOSC might be a potentially therapeutic compound for Alzheimer's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / antagonists & inhibitors*
  • Amyloid beta-Peptides / chemistry
  • Amyloid beta-Peptides / toxicity*
  • Animals
  • Apoptosis / drug effects
  • Calcium / metabolism
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Cerebral Cortex / drug effects
  • Humans
  • Lipid Peroxidation / drug effects
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Mitochondria / drug effects
  • Mitochondria / physiology
  • Peptide Fragments / antagonists & inhibitors
  • Peptide Fragments / chemistry
  • Peptide Fragments / toxicity
  • Phaeophyceae / chemistry*
  • Rats
  • Tetrazolium Salts
  • Thiazoles
  • Thiobarbituric Acid Reactive Substances / metabolism


  • Amyloid beta-Peptides
  • Peptide Fragments
  • Tetrazolium Salts
  • Thiazoles
  • Thiobarbituric Acid Reactive Substances
  • amyloid beta-protein (1-40)
  • amyloid beta-protein (25-35)
  • thiazolyl blue
  • Calcium