Polysaccharide extract isolated from ganoderma lucidum protects rat cerebral cortical neurons from hypoxia/reoxygenation injury

J Pharmacol Sci. 2004 Jun;95(2):294-8. doi: 10.1254/jphs.sc0040011.

Abstract

The effect of polysaccharide extract isolated from Ganoderma lucidum (Gl-PS) on rat cortical neuronal cultures exposed to hypoxia/reoxygenation (H/R) was studied in vitro. Gl-PS (1, 10, 100 microg/ml) increased neuron viability following H/R as measured by the inhibition of MTT reduction. Gl-PS also significantly reduced malondialdehyde content and reactive oxygen species production and increased the manganese superoxide dismutase (Mn-SOD) activity; furthermore, the translocation of nuclear factor-kappa B induced by H/R was blocked. These findings suggest that Gl-PS might be useful in treating H/R-induced oxidative stress and Mn-SOD might play a critical role in the neuroprotective effect of Gl-PS against H/R injury.

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Cells, Cultured
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / pathology*
  • Fluorescent Antibody Technique
  • Hypoxia, Brain / pathology*
  • Hypoxia, Brain / prevention & control*
  • Malondialdehyde / metabolism
  • Microscopy, Confocal
  • NF-kappa B
  • Neuroprotective Agents*
  • Oxidative Stress / drug effects
  • Oxygen / pharmacology
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Reishi / chemistry*
  • Superoxide Dismutase / metabolism
  • Tetrazolium Salts
  • Thiazoles

Substances

  • NF-kappa B
  • Neuroprotective Agents
  • Polysaccharides
  • Tetrazolium Salts
  • Thiazoles
  • Malondialdehyde
  • Superoxide Dismutase
  • thiazolyl blue
  • Oxygen