In 2003, CDC released Advancing HIV Prevention: New Strategies for a Changing Epidemic. One of the four strategies of this initiative is to expand routine, voluntary human immunodeficiency virus (HIV) testing. This report describes the results of a state-funded program in Massachusetts that offered HIV counseling, testing, and referral (HIV CTR) to patients entering one of four hospital-associated urgent care centers. Among the 3,068 patients tested, the program identified an HIV seroprevalence of 2.0%. The findings underscore the effectiveness of routine HIV CTR in HIV case identification.