Effect of Japanese horseradish oil on methane production and ruminal fermentation in vitro and in steers

J Anim Sci. 2004 Jun;82(6):1839-46. doi: 10.2527/2004.8261839x.

Abstract

The effects of alpha-cyclodextrin-horseradish oil complex (CD-HR) on methane production and ruminal fermentation were studied in vitro and in steers. In the in vitro study, diluted ruminal fluid (30 mL) was incubated anaerobically at 38 degrees C for 6 h with or without CD-HR, using cornstarch as substrate. The CD-HR was added at various concentrations (0, 0.17, 0.85 and 1.7 g/L). Treatment affected neither the pH of the medium nor the number of protozoa. Total VFA increased in a linear manner (P = 0.02), and NH3-N decreased quadratically (P = 0.04) as the concentration of CD-HR increased from 0.17 g/L to 1.7 g/L. Molar proportions of acetate decreased in a linear manner (P = 0.03), and propionate increased linearly (P = 0.008) with increasing concentrations of CD-HR. Production of methane was inhibited up to 90%, whereas accumulation of dihydrogen was increased 36-fold by 1.7 g/L of CD-HR supplementation relative to controls. The effect of CD-HR on methane production, ruminal fermentation and microbes, and digestibility was further investigated in vivo using four Holstein steers in a crossover design. The CD-HR supplement was mixed into the concentrate portion of a (1.5:1) Sudangrass hay plus concentrate mixture that was fed twice daily to the steers. Ruminal samples were collected 0, 2, and 5 h after the morning feeding. No effects of CD-HR supplementation on ruminal pH (P = 0.63) or protozoal numbers (P = 0.44) were observed. Molar proportion of acetate was decreased (P = 0.04) and propionate was increased (P = 0.005) by CD-HR treatment. Molar proportion of butyrate was increased (P = 0.05) in CD-HR-supplemented steers. Ruminal NH3-N was decreased (P = 0.05) by treatment. Blood plasma glucose concentration was increased (P = 0.02) and urea-N was decreased (P = 0.04) with CD-HR supplementation. Daily DMI was decreased (P = 0.04), and apparent digestibility of DM (P = 0.13), NDF (P = 0.14), and CP tended (P = 0.14) to be increased by treatment. Methane production was decreased (P = 0.03) by 19%, and the number of methanogens was also decreased (P = 0.03). Although N retention (P = 0.11), total viable bacteria (P = 0.15), and sulfate-reducing bacteria (P = 0.17) were not significantly altered by treatment, tendencies for increases were noted with CD-HR supplementation. The number of cellulolytic (P = 0.38) and acetogenic bacteria (P = 0.32) remained unchanged by treatment. These results indicate that CD-HR supplementation can be used to decrease methane production in steers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria, Anaerobic / metabolism*
  • Cattle / metabolism*
  • Cattle / microbiology
  • Cross-Over Studies
  • Cyclodextrins / administration & dosage
  • Cyclodextrins / pharmacology
  • Digestion
  • Dose-Response Relationship, Drug
  • Energy Intake
  • Eukaryota / drug effects
  • Eukaryota / growth & development
  • Fatty Acids, Volatile / biosynthesis
  • Fermentation / drug effects
  • Hydrogen-Ion Concentration
  • In Vitro Techniques
  • Male
  • Methane / metabolism*
  • Plant Oils / administration & dosage*
  • Plant Oils / pharmacology
  • Random Allocation
  • Rumen / chemistry
  • Rumen / metabolism
  • Rumen / microbiology*
  • Rumen / parasitology
  • Wasabia*

Substances

  • Cyclodextrins
  • Fatty Acids, Volatile
  • Plant Oils
  • Methane