The genetic structure and diversity of the A and B mating-type genes from the tropical oyster mushroom, Pleurotus djamor

Fungal Genet Biol. 2004 Aug;41(8):813-25. doi: 10.1016/j.fgb.2004.04.005.


In most heterothallic mushroom species, inbreeding is avoided by an incompatibility system determined by two loci each with multiple alleles (the A and B mating-type loci). In this study we investigated the genetic structure of the mating-type loci in the tropical oyster mushroom Pleurotus djamor using both positional cloning and degenerate PCR methods. DNA sequences from genomic regions cosegregating with the mating-type loci of P. djamor revealed homeodomain transcription factors (A) and pheromone receptors (B), suggesting the genetic basis for mating-type determination in P. djamor is the same as in the model mushroom species. Three pheromone receptors were detected in a single homokaryotic isolate of P. djamor. Only one pair of homeodomain genes was detected in the A mating-type region. It is hypothesized that the A mating-type locus of P. djamor is comprised of only one homeodomain pair, which may explain the lower number of A mating-type alleles relative to other mushroom species.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Gene Library
  • Genes, Fungal*
  • Genes, Mating Type, Fungal*
  • Genetic Variation*
  • Molecular Sequence Data
  • Phylogeny
  • Pleurotus / classification
  • Pleurotus / genetics*
  • Receptors, Pheromone / genetics
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics
  • Transcription Factors / physiology


  • Receptors, Pheromone
  • Transcription Factors