Innate murine B cells produce anti-disialosyl antibodies reactive with Campylobacter jejuni LPS and gangliosides that are polyreactive and encoded by a restricted set of unmutated V genes

J Neuroimmunol. 2004 Jul;152(1-2):98-111. doi: 10.1016/j.jneuroim.2004.04.002.


In Guillain-Barré syndrome following Campylobacter enteritis, anti-lipopolysaccharide antibodies cross-react with neural gangliosides, thereby precipitating autoimmune neuropathy. We examined the properties of 15 murine anti-LPS/ganglioside mAbs specific for NeuAc(alpha2-8)NeuAc-Gal disialosyl epitopes. Many mAbs displayed features of an innate B cell origin including polyreactivity (13/15), hybridoma CD5 mRNA expression (5/15), predominance of IgM (9/15) or IgG3 (3/6) isotype, low affinity, and utilisation of unmutated VH and VL VDJ rearrangements. Antibody specificity resided in highly selective V gene usage, with 6/15 mAbs being encoded by the VH7183.3b gene. These data indicate that neuropathogenic antiganglioside autoantibodies can arise from the natural autoantibody repertoire.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Affinity
  • Antibody Specificity*
  • Autoantibodies / immunology*
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Campylobacter jejuni / immunology*
  • Epitopes, B-Lymphocyte / immunology
  • Gangliosides / genetics
  • Gangliosides / immunology*
  • Immunoglobulin Class Switching
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology
  • Immunoglobulin M / genetics
  • Immunoglobulin M / immunology
  • Immunoglobulin Variable Region / genetics
  • Lipopolysaccharides / immunology*
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction


  • Antibodies, Monoclonal
  • Autoantibodies
  • Epitopes, B-Lymphocyte
  • Gangliosides
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulin Variable Region
  • Lipopolysaccharides