[Immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen]

Zhonghua Gan Zang Bing Za Zhi. 2004 Jun;12(6):338-40.
[Article in Chinese]

Abstract

Objectives: To observe immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen (HBcAg).

Methods: A new DNA vaccine (pSW3891/HBc) encoding for hepatitis B virus core antigen was constructed using plasmid pSW3891 which can be used in human. Control and experiment groups of Balb/c mice were immunized with pSW3891 or pSW3891/HBc by gene gun. Anti-HBc in sera of mice was tested by ELISA (enzyme linked immune sorbent assay). Specific cytotoxicity of cytotoxic T lymphocyte (CTL) of mice was detected by LDH release assay.

Results: pSW3891/HBc can express in 293T cell line effectively. Mice immunized with pSW3891/HBc showed strong anti-HBc response and specific high cytotoxicity of CTL.

Conclusion: pSW3891/HBc induced significantly humoral and cellular immune responses in Balb/c mice.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hepatitis B Antibodies / blood*
  • Hepatitis B Core Antigens / genetics
  • Hepatitis B Core Antigens / immunology*
  • Hepatitis B Vaccines / immunology*
  • Immunity, Cellular
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes, Cytotoxic / immunology
  • Vaccines, DNA / immunology*

Substances

  • Hepatitis B Antibodies
  • Hepatitis B Core Antigens
  • Hepatitis B Vaccines
  • Vaccines, DNA