Sphingolipid C4 hydroxylation influences properties of yeast detergent-insoluble glycolipid-enriched membranes

FEBS Lett. 2004 Jul 2;569(1-3):272-6. doi: 10.1016/j.febslet.2004.05.074.


Sphingoid base C4 hydroxylation is required for syringomycin E action on the yeast plasma membrane. Detergent-insoluble glycolipid-enriched membranes (DIGs) from a yeast strain lacking C4 hydroxylated sphingoid bases (sur2delta) are composed of linear membrane fragments instead of vesicular structures observed for wild-type DIGs, though they have similar lipid compositions and amounts of DIG marker proteins. Light-scattering bands collected from sur2delta after centrifugation of Triton X-100-treated cell lysates in continuous density gradients have lower buoyant densities than that of the wild-type. The results show that C4 hydroxylation influences the physical and structural properties of DIGs and suggest that syringomycin E interacts with lipid rafts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Fractionation
  • Cell Membrane / metabolism
  • Centrifugation, Density Gradient
  • Detergents
  • Glycolipids / metabolism*
  • Membrane Lipids / metabolism*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / physiology*
  • Solubility
  • Sphingolipids / metabolism*
  • Sphingolipids / pharmacology*


  • Detergents
  • Glycolipids
  • Membrane Lipids
  • Sphingolipids