Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats

Pharmacol Res. 2004 Sep;50(3):231-6. doi: 10.1016/j.phrs.2004.03.004.


This study was designed to examine the effects of squalene on tissue antioxidant status in isoproterenol-induced myocardial infarction in male albino rats. Levels of diagnostic marker enzymes [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine phosphokinase (CPK)] in plasma, lipid peroxides, reduced glutathione, and the activities of glutathione-dependent antioxidant enzymes [glutathione peroxidase (GPx) and glutathione-S-transferase (GST)] and antiperoxidative enzymes [catalase (CAT) and superoxide dismutase (SOD)] in the heart tissue of experimental groups of rats were determined. The prior administration of squalene at 2% level along with feed for 45 days significantly prevented the isoproterenol-induced elevation in the levels of diagnostic marker enzymes in plasma of experimental rats. Squalene also exerted an antioxidant effect against isoproterenol-induced myocardial infarction by blocking the induction of lipid peroxidation. A tendency to prevent the isoproterenol-induced alterations in the level of reduced glutathione and in the activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes was also observed. The cardioprotective effect of squalene might be ascribable to its antioxidant property and membrane stabilizing action.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use
  • Free Radicals / antagonists & inhibitors
  • Free Radicals / metabolism
  • Isoproterenol / toxicity*
  • Lipid Peroxidation / drug effects*
  • Lipid Peroxidation / physiology
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / drug therapy*
  • Myocardial Infarction / metabolism
  • Rats
  • Rats, Wistar
  • Sharks
  • Squalene / pharmacology
  • Squalene / therapeutic use*


  • Antioxidants
  • Free Radicals
  • Squalene
  • Isoproterenol