Separation and identification of compounds in Rhizoma chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry

Xueguo Chen; Liang Kong; Xingye Su; Hongjing Fu; Jianyi Ni; Ruihuan Zhao; Hanfa Zou

doi:10.1016/j.chroma.2004.04.002

Separation and identification of compounds in Rhizoma chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry

J Chromatogr A. 2004 Jun 25;1040(2):169-78. doi: 10.1016/j.chroma.2004.04.002.

Authors

Xueguo Chen¹, Liang Kong, Xingye Su, Hongjing Fu, Jianyi Ni, Ruihuan Zhao, Hanfa Zou

Affiliation

¹ National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 161, Zhongshan Road, Dalian 116011, China.

PMID: 15230523
DOI: 10.1016/j.chroma.2004.04.002

Abstract

A comprehensive two-dimensional liquid chromatographic separation system based on the combination of a CN column and an ODS column is developed for the separation of components in a traditional Chinese medicine (TCM) Rhizoma chuanxiong. Two columns are coupled by a two-position, eight-port valve equipped with two storage loops and controlled by a computer. The effluent is detected by both the diode array detector and atmospheric pressure chemical ionization (APCI) mass spectrometer. More than 52 components in the methanol extract of R. chuanxiong were resolved and 11 of them were preliminary identified according to their UV and mass spectra.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid / methods*
Drugs, Chinese Herbal / chemistry
Drugs, Chinese Herbal / isolation & purification*
Mass Spectrometry / methods*
Spectrophotometry, Ultraviolet

Substances

Drugs, Chinese Herbal