Acteoside protects endothelial cells against free radical-induced oxidative stress

J Pharm Pharmacol. 2004 Jun;56(6):743-8. doi: 10.1211/0022357023501.

Abstract

The protective effect of acteoside against membrane lipid oxidation and free radical-mediated impairment of endothelial function was investigated. Results showed that iron-mediated oxidative modification of the cell membrane in cultured bovine pulmonary endothelial cells (PAECs) was significantly attenuated by acteoside as measured by thiobarbituric acid-reactive substances (TBARS). Fenton's reagent (H2O2/Fe2+) was used to generate hydroxyl radicals (*OH) and induce oxidative stress. Acteoside not only effectively minimized the loss of cell viability induced by hydroxyl radicals in cultured endothelial cells but also countered the free radical-induced destruction of the endothelium-dependent relaxation to acetylcholine in rat aorta. Furthermore, acteoside showed a dose-dependent scavenging effect of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals and appeared to be the most efficient in comparison with the four reference compounds (alpha-tocopherol, vitamin C, probucol and resveratrol). These data suggested that acteoside protects the cell from oxidative stress and that scavenging of free radicals could be a key mechanism contributing to the cytoprotective effect of acteoside.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Aorta, Thoracic / drug effects
  • Aorta, Thoracic / physiology
  • Biphenyl Compounds
  • Cattle
  • Cell Death / drug effects
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / metabolism
  • Glucosides / pharmacology*
  • Hydrazines
  • In Vitro Techniques
  • Male
  • Membrane Lipids / metabolism*
  • Oxidative Stress*
  • Phenols / pharmacology*
  • Picrates
  • Pulmonary Artery / cytology
  • Rats
  • Rats, Sprague-Dawley
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Vasodilation / drug effects

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Glucosides
  • Hydrazines
  • Membrane Lipids
  • Phenols
  • Picrates
  • Thiobarbituric Acid Reactive Substances
  • acteoside
  • 1,1-diphenyl-2-picrylhydrazyl