Identification of novel keratinocyte-secreted peptides dermokine-alpha/-beta and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1

Genomics. 2004 Aug;84(2):384-97. doi: 10.1016/j.ygeno.2004.03.010.


We performed high-throughput in situ hybridization screening of sections of mouse epidermis using an equalized skin cDNA library as probes and identified a novel gene giving rise to two splicing variants, both of which are expressed in the spinous layer. This gene was mapped between two genes encoding keratinocyte-related peptides, suprabasin and keratinocyte differentiation-associated protein (Kdap), on human chromosome 19q13.1. These gene products appeared to carry functional signal sequences. We then designated these two splicing variants as dermokine-alpha and -beta. Northern blotting and quantitative RT-PCR revealed that dermokine-alpha/-beta, suprabasin, and Kdap were highly expressed in stratified epithelia. In mouse embryonic development, dermokine-alpha/-beta began to be expressed during the period of stratification. Also, in differentiating primary cultured human keratinocytes, transcription of dermokine-alpha/-beta, suprabasin, and Kdap was induced. These findings indicated that dermokine-alpha/-beta, suprabasin, and Kdap are secreted from the spinous layer of the stratified epithelia and that these genes form a novel gene complex on the chromosome.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation / metabolism
  • Aspartic Acid Endopeptidases / metabolism
  • Chromosomes, Human, Pair 19 / genetics*
  • Cloning, Molecular
  • Epidermis / metabolism
  • Exons / genetics
  • Gene Expression Profiling
  • Humans
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins
  • Keratinocytes / metabolism*
  • Mice
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Physical Chromosome Mapping
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment


  • Antigens, Differentiation
  • DMKN protein, human
  • Intercellular Signaling Peptides and Proteins
  • Proteins
  • RNA, Messenger
  • dermokine protein, mouse
  • suprabasin protein, mouse
  • Aspartic Acid Endopeptidases
  • Kdap protein, mouse

Associated data

  • GENBANK/AY622962
  • GENBANK/AY622963
  • GENBANK/AY622964
  • GENBANK/AY622965