Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii

Plant Physiol. 2004 Jul;135(3):1595-607. doi: 10.1104/pp.104.041400. Epub 2004 Jul 2.


Photosynthetic acclimation to CO2-limiting stress is associated with control of genetic and physiological responses through a signal transduction pathway, followed by integrated monitoring of the environmental changes. Although several CO2-responsive genes have been previously isolated, genome-wide analysis has not been applied to the isolation of CO2-responsive genes that may function as part of a carbon-concentrating mechanism (CCM) in photosynthetic eukaryotes. By comparing expression profiles of cells grown under CO2-rich conditions with those of cells grown under CO2-limiting conditions using a cDNA membrane array containing 10,368 expressed sequence tags, 51 low-CO2 inducible genes and 32 genes repressed by low CO2 whose mRNA levels were changed more than 2.5-fold in Chlamydomonas reinhardtii Dangeard were detected. The fact that the induction of almost all low-CO2 inducible genes was impaired in the ccm1 mutant suggests that CCM1 is a master regulator of CCM through putative low-CO2 signal transduction pathways. Among low-CO2 inducible genes, two novel genes, LciA and LciB, were identified, which may be involved in inorganic carbon transport. Possible functions of low-CO2 inducible and/or CCM1-regulated genes are discussed in relation to the CCM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acclimatization
  • Amino Acid Sequence
  • Animals
  • Carbon / metabolism*
  • Carbon Dioxide / metabolism
  • Carbon Dioxide / pharmacology*
  • Chlamydomonas reinhardtii / drug effects
  • Chlamydomonas reinhardtii / genetics*
  • Chlamydomonas reinhardtii / metabolism*
  • Conserved Sequence
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation / genetics
  • Humans
  • Molecular Sequence Data
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • Protozoan Proteins
  • Carbon Dioxide
  • Carbon

Associated data

  • GENBANK/AB168092
  • GENBANK/AB168093
  • GENBANK/AB168094