Effect of leptin on healing of colonic anastomoses in rats

Hepatogastroenterology. 2004 Jul-Aug;51(58):994-7.


Background/aims: Anastomotic leaks are continuing to be the source of major morbidity in colorectal surgery. Previous studies have shown that leptin acts as a growth factor for several cell types. The aim of this study was to evaluate the effect of leptin on healing of colonic anastomoses in rats.

Methodology: Forty-eight rats were divided into 5 groups. Group I (n=8) sham; group II (n=10) control; right colonic anastomosis, group III (n=10); following right colonic anastomosis, treated with leptin twice-daily 1 mg/kg intraperitoneally, group IV (n=10); before right colonic anastomosis, 45 min of colonic ischemia has been created, group V (n=10); following 45 min of colonic ischemia and right colonic anastomosis, leptin was given twice-daily 1 mg/kg intraperitoneally. On the 7th postoperative day relaparotomy was performed. Bursting pressure (BP), tissue hydroxyproline concentrations (THPC), and histopathologic properties of anastomoses; vascular tissue proliferation (VTP), collagen tissue proliferation (CTP), polymorphonuclear leukocyte infiltration (PMNLI), mononuclear leukocyte infiltration (MNLI) were analyzed and results were compared statistically.

Results: BP and THPC were found to be significantly higher in group III and group V in comparison with group II and group IV respectively (P<0.05). Histopathologically, leptin significantly increased VTP, CTP, MNLI (P<0.001), and significantly decreased PMNLI (p<0.05) on non-ischemic and ischemic colonic anastomoses.

Conclusions: Leptin can be used safely in colorectal surgery since it accelerates the healing of colonic anastomoses.

MeSH terms

  • Anastomosis, Surgical*
  • Animals
  • Blood Vessels / pathology
  • Cell Division
  • Collagen / metabolism
  • Colon / blood supply
  • Colon / metabolism
  • Colon / pathology
  • Colon / physiopathology*
  • Colon / surgery*
  • Hydroxyproline / metabolism
  • Ischemia / physiopathology
  • Leptin / pharmacology*
  • Male
  • Neutrophil Infiltration
  • Rats
  • Rats, Wistar
  • Tensile Strength
  • Wound Healing / drug effects*


  • Leptin
  • Collagen
  • Hydroxyproline