Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP

Biochem Biophys Res Commun. 2004 Jul 30;320(3):760-5. doi: 10.1016/j.bbrc.2004.06.022.


Daxx-mediated transcriptional repression was modulated by a speckled POZ domain protein SPOP which was first identified as an autoantigen from the serum of a scleroderma patient. This is the first report on the biochemical and functional interactions between Daxx and SPOP. The COOH-terminal region of Daxx interacts with the NH2-terminal region of SPOP. SPOP reversed the transcriptional repression mediated by Daxx which binds with ETS1 transcription factor to repress ETS1-responsive gene expression. Mutagenesis study suggests that the ability of SPOP to self-associate as well as its ability to bind with Daxx was important for the modulation of Daxx-mediated transcriptional repression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Cell Line
  • Chlorocebus aethiops
  • Gene Expression Regulation / physiology
  • Humans
  • Kidney / metabolism*
  • Matrix Metalloproteinase 1 / genetics
  • Matrix Metalloproteinase 1 / metabolism*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Repressor Proteins
  • Structure-Activity Relationship
  • Transcription, Genetic / genetics*


  • Nuclear Proteins
  • Repressor Proteins
  • SPOP protein, human
  • Matrix Metalloproteinase 1