Synergistic effect on insulin secretion from INS-1 cells of a sulfonylurea and a phosphodiesterase 3 inhibitor

Life Sci. 2004 Aug 6;75(12):1479-90. doi: 10.1016/j.lfs.2004.03.015.

Abstract

The effects on insulin secretion from INS-1 cells of varying concentrations of the sulfonylurea glyburide and the PDE3 inhibitor milrinone, separately and in combination were measured. Over a range of concentrations the effects of the two drugs in combination were more than additive. A response surface model was fit to the data and was found to describe the data well. From this model, it was apparent that a significant synergistic effect upon insulin secretion existed over a wide range of combinations of the two drugs.

Publication types

  • Comparative Study

MeSH terms

  • 3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors*
  • Animals
  • Cells, Cultured
  • Cyclic Nucleotide Phosphodiesterases, Type 3
  • Dose-Response Relationship, Drug
  • Drug Synergism
  • Glyburide / pharmacology*
  • Insulin / metabolism*
  • Insulin Secretion
  • Islets of Langerhans / drug effects*
  • Islets of Langerhans / metabolism
  • Milrinone / pharmacology*
  • Models, Biological
  • Radioimmunoassay
  • Rats

Substances

  • Insulin
  • 3',5'-Cyclic-AMP Phosphodiesterases
  • Cyclic Nucleotide Phosphodiesterases, Type 3
  • Milrinone
  • Glyburide