Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli

Kannika Sajjaphan; Nir Shapir; Lawrence P Wackett; Michael Palmer; Barbara Blackmon; Jeff Tomkins; Michael J Sadowsky

doi:10.1128/AEM.70.7.4402-4407.2004

Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli

Appl Environ Microbiol. 2004 Jul;70(7):4402-7. doi: 10.1128/AEM.70.7.4402-4407.2004.

Authors

Kannika Sajjaphan¹, Nir Shapir, Lawrence P Wackett, Michael Palmer, Barbara Blackmon, Jeff Tomkins, Michael J Sadowsky

Affiliation

¹ Department of Soil, Water, and Climate, Center for Microbial and Plant Genomics, 439 Borlaug Hall, 1991 Upper Buford Cir., University of Minnesota, St. Paul, MN 55108, USA.

Abstract

Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Arthrobacter / genetics*
Atrazine / metabolism*
Base Sequence
Chromosomes, Artificial, Bacterial
Escherichia coli / genetics*
Genes, Bacterial*
Genetic Linkage*
Molecular Sequence Data
Plasmids

Substances

Atrazine

Associated data

GENBANK/AY456696