[Nanoparticulate silver. A new antimicrobial substance for bone cement]

Orthopade. 2004 Aug;33(8):885-92. doi: 10.1007/s00132-004-0690-8.
[Article in German]


Background: Multiresistant bacteria have become an important problem in prosthetic joint infections. Their frequent resistance against gentamicin, which is commonly used in antibiotic-loaded bone cements, makes a new prophylaxis necessary.

Methods: PMMA-cement was loaded with 1% nanoparticulate silver and its antibacterial activity tested in vitro against gentamicin-resistant MRSE and MRSA strains as well as being compared to the activity of plain and gentamicin-loaded bone cements. A quantitative elution testing was also done to study the potentially cytotoxic effects of NanoSilver cement.

Results: Unloaded and PMMA-cement loaded with 2% gentamicin did not exhibit any antibacterial activity against MRSE and MRSA. At 1%, NanoSilver cement completely inhibited the proliferation of MRSA and MRSE. NanoSilver bone cement did not show any significant differences compared to the non-toxic control group.

Conclusions: If these promising in vitro results can be confirmed in vivo, NanoSilver bone cement may be of considerable value in total joint arthroplasty.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Anti-Bacterial Agents / toxicity
  • Bone Cements / pharmacology*
  • Bone Cements / toxicity*
  • Cell Line
  • Cell Survival / drug effects*
  • Dose-Response Relationship, Drug
  • Drug Resistance, Multiple*
  • Fibroblasts / drug effects
  • Gentamicins / pharmacology
  • Gentamicins / toxicity
  • Methicillin Resistance*
  • Mice
  • Microbial Sensitivity Tests
  • Microscopy, Electron, Scanning
  • Nanotechnology*
  • Particle Size
  • Polymethyl Methacrylate / pharmacology*
  • Polymethyl Methacrylate / toxicity
  • Staphylococcus aureus / drug effects*
  • Staphylococcus epidermidis / drug effects*


  • Anti-Bacterial Agents
  • Bone Cements
  • Gentamicins
  • Polymethyl Methacrylate