CiYB1 is a major component of storage mRNPs in ascidian oocytes: implications in translational regulation of localized mRNAs

Dev Biol. 2004 Aug 1;272(1):217-30. doi: 10.1016/j.ydbio.2004.04.032.

Abstract

In ascidian eggs, the existence of several localized maternal cytoplasmic determinants has been proposed and the importance of localized mRNAs for tissue differentiation has been demonstrated. We previously identified the ascidian Y-box proteins (CiYB1, 2 and 3), homologues of which are known to be involved in the storage of maternal mRNA in oocytes of other organisms. In this study, we found that CiYB1 protein is abundant in the gonad, egg, and embryo. Purification of messenger ribonucleoprotein (mRNP) particles from the gonad revealed that CiYB1 was one of their major components. A significant change in the distribution of CiYB1 protein from stored mRNP particles in the gonad to the ribosome fraction in eggs and embryos was observed. This change correlates most likely with the shift of stored maternal mRNAs to polyribosomes. Moreover, we found that CiYB1 colocalized with Cipem and Ci-macho1 mRNAs, which are localized at the posterior end of the embryo at the cleavage stage. Cipem and Ci-macho1 mRNAs were co-immunoprecipitated with CiYB1 in the oocyte and embryo lysates. The formation of a complex between Cipem mRNA and CiYB1 protein resulted in translational repression in the in vitro translation system. Our results indicate that associating with CiYB1 protein contributes to the translational control of the localized mRNA in eggs and embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Ciona intestinalis / embryology
  • Ciona intestinalis / genetics*
  • Ciona intestinalis / metabolism
  • Cytoplasm / metabolism
  • Egg Proteins / genetics
  • Egg Proteins / metabolism
  • Embryo, Nonmammalian
  • Female
  • Gene Expression Regulation
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism*
  • Intracellular Signaling Peptides and Proteins*
  • Macromolecular Substances
  • Molecular Sequence Data
  • Oocytes / physiology*
  • Protein Biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism*
  • Zygote / metabolism

Substances

  • Carrier Proteins
  • Egg Proteins
  • Heat-Shock Proteins
  • Intracellular Signaling Peptides and Proteins
  • Macromolecular Substances
  • RNA, Messenger
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Y-box protein, Ciona intestinalis
  • macho-1 protein, Halocynthia roretzi
  • messenger ribonucleoprotein