Characterization of ciliated bronchial epithelium 1, a ciliated cell-associated gene induced during mucociliary differentiation

Am J Respir Cell Mol Biol. 2004 Nov;31(5):491-500. doi: 10.1165/rcmb.2004-0050OC. Epub 2004 Jul 8.


Lung epithelial structure is altered in asthma; however, the precise mechanisms underlying epithelial repair, including differentiation from basal to columnar epithelial cells, are not well defined. In the course of random sequencing of a cDNA library from human lung biopsies, we have identified a novel gene, ciliated bronchial epithelium 1 (CBE1). Expression of CBE1 was induced during in vitro differentiation of bronchial epithelial cells. Synchronous expression with tektin and hepatocyte nuclear factor 3/forkhead homologue 4, down-regulation by interleukin-13, and its tissue distribution strongly suggested that CBE1 is associated with ciliated cells. Two isoforms of the 0.7-kb full-length cDNA were identified, resulting in open reading frames with different carboxyl termini, with no homology to known proteins. Expression of CBE1 in ciliated epithelial cells was confirmed by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction analysis using bronchial biopsies showed no difference of expression of CBE1 between normal subjects and subjects with asthma. Expression studies showed that CBE1 is nuclear- or perinuclear-localized, depending on cell type. Regulated expression during differentiation and the subcellular localization of CBE1 suggest that it may play an important role in the differentiation and/or function of ciliated cells in human airways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Asthma / metabolism
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Bronchi / cytology*
  • COS Cells
  • Cell Differentiation
  • Cell Line
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Cloning, Molecular
  • DNA Mutational Analysis
  • DNA Primers / chemistry
  • DNA, Complementary / metabolism
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism
  • Down-Regulation
  • Epithelial Cells / cytology*
  • Forkhead Transcription Factors
  • Gene Library
  • Hepatocyte Nuclear Factor 3-alpha
  • Humans
  • Immunohistochemistry
  • Interleukin-13 / metabolism
  • Lung / cytology
  • Lung / pathology
  • Microscopy, Confocal
  • Microtubule Proteins / metabolism
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / metabolism
  • Open Reading Frames
  • Plasmids / metabolism
  • Protein Structure, Tertiary
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution
  • Trans-Activators / chemistry
  • Transcription Factors / biosynthesis
  • Transcription Factors / chemistry*
  • Transcription Factors / metabolism


  • C9orf24 protein, human
  • DNA Primers
  • DNA, Complementary
  • DNA-Binding Proteins
  • FOXA1 protein, human
  • FOXJ1 protein, human
  • Forkhead Transcription Factors
  • Hepatocyte Nuclear Factor 3-alpha
  • Interleukin-13
  • Microtubule Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • Trans-Activators
  • Transcription Factors
  • tektins