World-wide survey of an Accord insertion and its association with DDT resistance in Drosophila melanogaster

Mol Ecol. 2004 Aug;13(8):2491-504. doi: 10.1111/j.1365-294X.2004.02263.x.


Previous work showed that insecticide resistance in Drosophila melanogaster is correlated with the insertion of an Accord-like element into the 5' region of the cytochrome P450 gene, Cyp6g1. Here, we study the distribution of the Accord-like element in 673 recently collected D. melanogaster lines from 34 world-wide populations. We also examine the extent of microsatellite variability along a 180-kilobase (kb) genomic region of chromosome II encompassing the resistance gene. We confirm a 100% correlation of the Accord insertion with insecticide resistance and a significant reduction in variability extending at least 20 kb downstream of the Cyp6g1 gene. The frequency of the Accord insertion differs significantly between East African (32-55%) and nonAfrican (85-100%) populations. This pattern is consistent with a selective sweep driving the Accord insertion close to fixation in nonAfrican populations as a result of the insecticide resistance phenotype it confers. This study confirms that hitchhiking mapping can be used to identify beneficial mutations in natural populations.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Cytochrome P-450 Enzyme System / genetics*
  • DDT / poisoning*
  • DNA Primers
  • DNA Transposable Elements / genetics*
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / drug effects
  • Drosophila melanogaster / genetics*
  • Drug Resistance / genetics*
  • Electrophoresis, Agar Gel
  • Gene Frequency
  • Genetic Carrier Screening
  • Genetic Variation
  • Microsatellite Repeats / genetics
  • Mutation / genetics
  • Polymerase Chain Reaction
  • Selection, Genetic*


  • DNA Primers
  • DNA Transposable Elements
  • Drosophila Proteins
  • Cytochrome P-450 Enzyme System
  • DDT
  • Cyp6g1 protein, Drosophila