The aging/precancerous gastric mucosa: a pilot nutraceutical trial

Ann N Y Acad Sci. 2004 Jun:1019:195-9. doi: 10.1196/annals.1297.031.


The aim of this study was to test the effect of antioxidant supplementation on enzymatic abnormalities and free radical-modified DNA adducts associated with premalignant changes in the gastric mucosa of elderly patients with HP-negative atrophic gastritis (CAG). Sixty patients with atrophic gastritis and intestinal metaplasia underwent a nutritional interview and a gastroscopy with multiple biopsy samples in the antrum that were processed for histology and for assaying: alpha-tocopherol, MDA, xanthine oxidase (XO), ornithine decarboxylase (ODC), and 8-OHdG. Patients were randomly allocated into three matched groups and supplemented for 6 months with (1) vitamin E, 300 mg/day; (2) multivitamin, two tablets t.i.d.; and (3) Immun-Age 6 g/day nocte (ORI, Gifu, Japan), a certified fermented papaya preparation with basic science-validated antioxidant/immunomodulant properties. Ten dyspeptic patients served as controls. Histology and biochemistry were blindly repeated at 3 and 6 months. CAG patients showed a significantly (P <.05) increased level of mucosal MDA and XO concentration that were reverted to normal by each supplementation (P <.05). All supplements caused a significant decrease of ODC (P <.01), but Immun-Age yielded the most effective (P < 0.05) and was the only one significantly decreasing 8-OhdG (P < 0.05). These data suggest that antioxidant supplementation, and, namely, Immun-Age, might be potential chemopreventive agents in HP-eradicated CAG patients and especially in the elderly population.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aging*
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • DNA / metabolism
  • DNA Adducts
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / metabolism
  • Dietary Supplements
  • Gastric Mucosa / pathology*
  • Gastritis
  • Gastritis, Atrophic / pathology*
  • Gene Expression Regulation
  • Humans
  • Models, Biological
  • Mucous Membrane / pathology
  • Oxidants / metabolism
  • Oxidative Stress
  • Pilot Projects
  • Precancerous Conditions
  • Random Allocation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stomach Neoplasms / pathology*
  • Up-Regulation
  • Vitamin E / therapeutic use
  • Vitamins / metabolism
  • Xanthine Oxidase / metabolism


  • Antioxidants
  • DNA Adducts
  • Oxidants
  • Vitamins
  • Vitamin E
  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA
  • Xanthine Oxidase
  • Deoxyguanosine