The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans

Nat Cell Biol. 2004 Aug;6(8):777-83. doi: 10.1038/ncb1154. Epub 2004 Jul 11.


Temporal control of cell division is critical for proper animal development. To identify mechanisms involved in developmental arrest of cell division, we screened for cell-cycle mutants that disrupt the reproducible pattern of somatic divisions in the nematode C. elegans. Here, we show that the cdc-14 phosphatase is required for the quiescent state of specific precursor cells. Whereas budding yeast Cdc14p is essential for mitotic exit, inactivation of C. elegans cdc-14 resulted in extra divisions in multiple lineages, with no apparent defects in mitosis or cell-fate determination. CDC-14 fused to the green fluorescent protein (GFP-CDC-14) localized dynamically and accumulated in the cytoplasm during G1 phase. Genetic interaction and transgene expression studies suggest that cdc-14 functions upstream of the cki-1 Cip/Kip inhibitor to promote accumulation of CKI-1 in the nucleus. Our data support a model in which CDC-14 promotes a hypophosphorylated and stable form of CKI-1 required for developmentally programmed cell-cycle arrest.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Caenorhabditis elegans / cytology*
  • Caenorhabditis elegans / embryology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Cycle
  • Cell Division
  • Cell Lineage
  • Codon, Terminator
  • Cytoplasm / chemistry
  • Exons
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Hot Temperature
  • Luminescent Proteins / metabolism
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / chemistry
  • Phosphoprotein Phosphatases / genetics*
  • Phosphoprotein Phosphatases / metabolism*
  • Protein Structure, Tertiary
  • RNA Interference
  • Recombinant Fusion Proteins / metabolism
  • Sequence Deletion


  • Caenorhabditis elegans Proteins
  • Codon, Terminator
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Phosphoprotein Phosphatases

Associated data

  • GENBANK/AY661743
  • GENBANK/AY661744
  • GENBANK/AY661745
  • GENBANK/AY661746
  • GENBANK/AY661747