Dynamics of CaCdc10, a septin of Candida albicans, in living cells and during infection

Int Microbiol. 2004 Jun;7(2):105-12.

Abstract

The morphogenetic program in the pathogenic fungus Candida albicans, including the dimorphic transition, is an interesting field of study, not only because it is absent in the commonly used model yeast Saccharomyces cerevisiae, but because of the close relationship between hyphal development and virulence of C. albicans. We studied one of the most important aspects of fungal morphogenesis--the septin ring--in C. albicans. By using a fusion construct to green fluorescent protein (GFP), the subcellular localization and dynamics of C. albicans Cdc10 in the different morphologies that this fungus is able to adopt was identified. The localization features reached were contrasted and compared with the results obtained from Candida cells directly extracted from an animal infection model under environmental conditions as similar as possible to the physiological conditions encountered by C. albicans during host infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Candida albicans / chemistry*
  • Candida albicans / cytology
  • Candida albicans / metabolism
  • Candidiasis / microbiology*
  • Cell Cycle
  • Cell Cycle Proteins / analysis*
  • Cell Cycle Proteins / genetics
  • Fungal Proteins / analysis*
  • Fungal Proteins / genetics
  • GTP Phosphohydrolases
  • Green Fluorescent Proteins
  • Hyphae / growth & development
  • Luminescent Proteins / genetics
  • Membrane Proteins
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred DBA
  • Microscopy, Fluorescence
  • Morphogenesis / physiology
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Septins
  • Transcription Factors

Substances

  • Cell Cycle Proteins
  • Fungal Proteins
  • Luminescent Proteins
  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • cdc10 protein, S pombe
  • Green Fluorescent Proteins
  • CDC10 protein, S cerevisiae
  • GTP Phosphohydrolases
  • Sept7 protein, mouse
  • Septins