Localized membrane depolarizations and localized calcium influx during electric field-guided neurite growth

Neuron. 1992 Sep;9(3):393-403. doi: 10.1016/0896-6273(92)90178-g.

Abstract

Our study explores the mechanisms behind neurite galvanotropism. Using phase, differential interference contrast and ratiometric fluorescence microscopy, we reveal four responses of N1E-115 mouse neuroblastoma cells to 0.1-1.0 mV/microns uniform DC electric fields: cathode-directed neurite initiation and elongation, cathode-biased growth cone filopodial protrusions, transient cathode-localized calcium increases, and persistent cathode-localized membrane depolarizations. These newly demonstrated events are temporally and spatially correlated, suggesting that they are causally related. The calcium increases are prevented by calcium channel blockers and by the removal of extracellular calcium. We therefore propose that the observed field-induced membrane depolarizations activate voltage-dependent calcium channels, resulting in cathode-localized calcium influx. This, in turn, may initiate the observed cathode-biased growth cone filopodial protrusions, followed by the cathode-directed neurite elongation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Calcium Channel Blockers / pharmacology
  • Cell Membrane / physiology
  • Electrophysiology / methods
  • Intracellular Membranes / metabolism
  • Membrane Potentials
  • Microscopy, Interference
  • Neurites / physiology*
  • Osmolar Concentration
  • Pyridinium Compounds
  • Tissue Distribution
  • Tumor Cells, Cultured

Substances

  • Calcium Channel Blockers
  • Pyridinium Compounds
  • Calcium