Abstract
Zoocin A is a streptococcolytic enzyme produced by Streptococcus equi subsp. zooepidemicus 4881 that has an unknown site of action on the peptidoglycans of susceptible organisms. Analysis of a mutant strain in which the genes for zoocin A and resistance to zoocin A were inactivated revealed that this strain was more susceptible to beta-lactam antibiotics than the parental organism. Purified zoocin A had weak beta-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic activity was inhibited by penicillin. Thus, zoocin A is a penicillin-binding protein and presumably is a D-alanyl endopeptidase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism*
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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Endopeptidases / genetics
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Endopeptidases / isolation & purification
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Endopeptidases / metabolism*
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Gene Deletion
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Genes, Bacterial
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Molecular Sequence Data
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Mutation
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Penicillin-Binding Proteins / genetics
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Penicillin-Binding Proteins / metabolism*
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Sequence Analysis, DNA
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Streptococcus equi / enzymology*
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Streptococcus equi / genetics
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beta-Lactam Resistance
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beta-Lactamases / genetics
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beta-Lactamases / isolation & purification
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beta-Lactamases / metabolism
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beta-Lactams / metabolism
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beta-Lactams / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Penicillin-Binding Proteins
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ZooA protein, Streptococcus equi
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beta-Lactams
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Endopeptidases
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beta-Lactamases
Associated data
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GENBANK/AY536057
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GENBANK/AY536058