Prostaglandin production by melanocytic cells and the effect of alpha-melanocyte stimulating hormone

FEBS Lett. 2004 Jul 16;570(1-3):223-6. doi: 10.1016/j.febslet.2004.06.041.

Abstract

Prostaglandins are potent mediators of the inflammatory response and are also involved in cancer development. In this study, we show that human melanocytes and FM55 melanoma cells express cyclooxygenase-1 and -2 (COX-1 and -2) and thus have the capability to produce prostaglandins. The FM55 cells produced predominantly PGE2 and PGF2alpha, whereas the HaCaT keratinocyte cell line produced mainly PGE2. The anti-inflammatory peptide, alpha-melanocyte stimulating hormone (alpha-MSH), reduced prostaglandin production in FM55 and HaCaT cells and reversed the effect of the pro-inflammatory cytokine TNF-alpha in the former. These results indicate that melanocytes produce prostaglandins and that alpha-MSH, by inhibiting this response, may play an important role in regulating inflammatory responses in the skin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Survival
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Humans
  • Inflammation
  • Isoenzymes / metabolism
  • Keratinocytes / metabolism
  • Melanocytes / metabolism*
  • Membrane Proteins
  • Phenotype
  • Prostaglandin-Endoperoxide Synthases / metabolism
  • Prostaglandins / biosynthesis*
  • Prostaglandins / metabolism
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Necrosis Factor-alpha / metabolism
  • alpha-MSH / metabolism*

Substances

  • Isoenzymes
  • Membrane Proteins
  • Prostaglandins
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • alpha-MSH
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • PTGS1 protein, human
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases