Abstract
An approach for isolation of cell surface, nucleic acid binding proteins is described. This approach relies on affinity modification of the proteins of living cells with reactive oligonucleotides bearing a haptenic group. Covalently modified proteins were isolated by hapten-specific affinity chromatography with subsequent SDS-PAGE. Isolated 68-kDa proteins responsible for the binding of oligonucleotides were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e, and albumin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoma, Squamous Cell / pathology
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Cell Line, Tumor
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Chromatography, Affinity
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DNA-Binding Proteins / isolation & purification*
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DNA-Binding Proteins / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Haptens / chemistry
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Humans
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Keratin-10
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Keratin-2
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Keratins / chemistry
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Keratins / isolation & purification
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Keratins / metabolism
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Mass Spectrometry
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Molecular Weight
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Neoplasms, Squamous Cell / pathology
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Oligodeoxyribonucleotides / chemical synthesis
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / isolation & purification*
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Oligodeoxyribonucleotides / metabolism*
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Sequence Analysis, Protein
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Serum Albumin / isolation & purification
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Serum Albumin / metabolism
Substances
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DNA-Binding Proteins
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Haptens
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KRT10 protein, human
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KRT2 protein, human
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Keratin-2
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Oligodeoxyribonucleotides
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Serum Albumin
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Keratin-10
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Keratins