Subcellular localization of frizzled receptors, mediated by their cytoplasmic tails, regulates signaling pathway specificity

PLoS Biol. 2004 Jul;2(7):E158. doi: 10.1371/journal.pbio.0020158. Epub 2004 Jul 13.

Abstract

The Frizzled (Fz; called here Fz1) and Fz2 receptors have distinct signaling specificities activating either the canonical Wnt/beta-catenin pathway or Fz/planar cell polarity (PCP) signaling in Drosophila. The regulation of signaling specificity remains largely obscure. We show that Fz1 and Fz2 have different subcellular localizations in imaginal disc epithelia, with Fz1 localizing preferentially to apical junctional complexes, and Fz2 being evenly distributed basolaterally. The subcellular localization difference directly contributes to the signaling specificity outcome. Whereas apical localization favors Fz/PCP signaling, it interferes with canonical Wnt/beta-catenin signaling. Receptor localization is mediated by sequences in the cytoplasmic tail of Fz2 that appear to block apical accumulation. Based on these data, we propose that subcellular Fz localization, through the association with other membrane proteins, is a critical aspect in regulating the signaling specificity within the Wnt/Fz signaling pathways.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning
  • Cadherins / metabolism
  • Cell Membrane / metabolism
  • Crosses, Genetic
  • Cytoplasm / metabolism*
  • Drosophila
  • Drosophila Proteins / biosynthesis
  • Drosophila Proteins / metabolism
  • Drosophila Proteins / physiology*
  • Frizzled Receptors
  • Immunohistochemistry
  • Ligands
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / physiology*
  • Microscopy, Fluorescence
  • Mutation
  • Phenotype
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptors, G-Protein-Coupled
  • Receptors, Neurotransmitter / biosynthesis
  • Receptors, Neurotransmitter / physiology*
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction
  • Transgenes
  • Wnt Proteins / metabolism
  • beta Catenin / metabolism

Substances

  • Cadherins
  • Drosophila Proteins
  • Frizzled Receptors
  • Ligands
  • Membrane Proteins
  • Receptors, G-Protein-Coupled
  • Receptors, Neurotransmitter
  • Recombinant Fusion Proteins
  • Wnt Proteins
  • beta Catenin
  • fz protein, Drosophila
  • fz2 protein, Drosophila
  • stan protein, Drosophila