High-throughput cloning of Campylobacter jejuni ORfs by in vivo recombination in Escherichia coli

J Proteome Res. May-Jun 2004;3(3):582-6. doi: 10.1021/pr0341134.

Abstract

A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning approach based on recombination in E. coli. The approach uses native E. coli recombination functions and requires no in vitro enzymatic steps or special strains. Our results indicate that this approach is an efficient and economical alternative for high-throughput cloning.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Campylobacter jejuni / genetics*
  • Escherichia coli / genetics*
  • Open Reading Frames / genetics*
  • Plasmids / genetics*
  • Recombination, Genetic*