Optical projection tomography

Annu Rev Biomed Eng. 2004;6:209-28. doi: 10.1146/annurev.bioeng.6.040803.140210.


Optical projection tomography is a new approach for three-dimensional (3-D) imaging of small biological specimens. It fills an imaging gap between MRI and confocal microscopy, being most suited to specimens that are from 1 to 10 mm across. The tomographic principles of optical projection tomography (OPT) are explained, its most important applications in biomedical research explored, and comparisons drawn of its pros and cons compared to a number of alternative imaging technologies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Gene Expression
  • Humans
  • Image Processing, Computer-Assisted
  • Microscopy, Confocal
  • Tomography, Optical / instrumentation
  • Tomography, Optical / methods*