Roles of the AtErcc1 protein in recombination

Plant J. 2004 Aug;39(3):334-42. doi: 10.1111/j.1365-313X.2004.02136.x.

Abstract

Summary Atercc1, the recently characterized Arabidopsis homologue of the Ercc1 (Rad10) protein, is a key component of nucleotide excision repair as part of a structure-specific endonuclease which cleaves 5' to UV photoproducts in DNA. This endonuclease also acts in removing overhanging non-homologous DNA 'tails' in synapsed recombination intermediates. We have previously demonstrated this recombination function of the Arabidopsis thaliana Xpf homologue, AtRad1p, and show here that recombination between plasmid DNA substrates containing non-homologous tails is specifically reduced 12-fold in atercc1 mutant plants compared with the wild type. Furthermore, using chromosomal tandem-repeat recombination substrates, we show that AtErcc1p is required for bleomycin induction of mitotic recombination in the chromosomal context. This work thus confirms both the specific and general recombination roles of the Atercc1 protein in recombination in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Base Sequence
  • DNA Repair
  • DNA, Bacterial / genetics
  • DNA, Plant / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Endonucleases / genetics
  • Endonucleases / metabolism*
  • Genes, Plant
  • Mutation
  • Plants, Genetically Modified
  • Recombination, Genetic*

Substances

  • Arabidopsis Proteins
  • DNA, Bacterial
  • DNA, Plant
  • DNA-Binding Proteins
  • T-DNA
  • Endonucleases
  • Ercc1 protein, Arabidopsis