[Effect of XK469 and adriamycin on the growth of H460 cells in vitro and its mechanism]

Di Yi Jun Yi Da Xue Xue Bao. 2004 Jul;24(7):775-8.
[Article in Chinese]

Abstract

Objective: To investigate the inhibitory effect of XK469 on the in vitro growth of H460 cells and its mechanism.

Methods: The survival curves of H460 cells treated with XK469, XN472 and adriamycin, respectively, were obtained by MTT analysis, and the effect of XK469 and adriamycin on the cell cycle of H460 cells examined by flow cytometry. Western blotting was adopted for detecting the expression of cdc2 and phos-cdc2 induced by XK469 and adriamycin.

Results: Different concentrations of XK469 and adriamycin could significantly inhibit the growth of H460 cells, induce their G2/M phase arrest, and increase phos-cdc2 expression; XN472 had a lesser effect on the growth of H460 cells.

Conclusion: XK469 can increase phos-cdc2 expression and induce G2/M phase cell cycle arrest of H460 cells, resulting in inhibition of H460 cell growth. The inhibitory effect of XK469 on H460 cell growth is attributed to the chlorine in the 7-positon of its structure.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • CDC2 Protein Kinase / metabolism
  • Carcinoma, Non-Small-Cell Lung / drug therapy*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Cell Cycle / drug effects
  • Cell Cycle Proteins / metabolism
  • Cell Division / drug effects
  • Doxorubicin / pharmacology*
  • Humans
  • Lung Neoplasms / drug therapy*
  • Lung Neoplasms / pathology
  • Quinoxalines / pharmacology*
  • cdc25 Phosphatases / metabolism

Substances

  • Cell Cycle Proteins
  • Quinoxalines
  • XK 469
  • Doxorubicin
  • CDC2 Protein Kinase
  • CDC25C protein, human
  • cdc25 Phosphatases