[Preparation and characterization of podophyllotoxin-dipalmitoylphosphatidylcholine proliposome]

Di Yi Jun Yi Da Xue Xue Bao. 2004 Jul;24(7):784-6, 788.
[Article in Chinese]

Abstract

Objective: To prepare podophyllotoxin-dipalmitoylphosphatidylcholine (PPT-DPPC) proliposomes (PPT-DPPC-PL) for improvement of the stability of PPT-DPPC liposome.

Methods: Freeze-drying method was used to prepare PPT-DPPC-PL, and the particle morphology, size range, encapsulation efficiency and stability of PPT-DPPC liposome were investigated.

Results: After hydration of PPT-DPPC-PL, PPT-DPPC liposome appeared multivesicular under electron microscope and the particles were distributed homogeneously with an average particle size of 1.45+/-0.38 microm. The encapsulation efficiency of PPT was 72.3%, and after storage at 4 to 40 degrees Celsius; for 1 to 6 months, the proliposome remained stable.

Conclusion: The prepared PPT-DPPC-PL particles by freeze-drying method are evenly distributed. The preparation method is relatively simple with higher embedding ratio and better stability.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1,2-Dipalmitoylphosphatidylcholine / administration & dosage*
  • Drug Stability
  • Freeze Drying
  • Liposomes
  • Particle Size
  • Podophyllotoxin / administration & dosage*
  • Temperature

Substances

  • Liposomes
  • 1,2-Dipalmitoylphosphatidylcholine
  • Podophyllotoxin