Binding of matrix attachment regions to lamin B1

Cell. 1992 Sep 18;70(6):949-59. doi: 10.1016/0092-8674(92)90245-8.

Abstract

Eukaryotic chromatin is organized into topologically constrained loops that are attached to the nuclear matrix. The regions of DNA that interact with the matrix are called matrix attachment regions (MARs). We studied the spatial distribution of MAR-binding sites in the nuclear matrix from rat liver cells, following a combined biochemical and ultrastructural approach. We found that MAR-binding sites are distributed equally over the internal fibrogranular network and the peripheral nuclear lamina. Internal and peripheral binding sites have similar binding characteristics: both sets of binding sites show specific and saturable binding of MARs from different organisms. By means of a DNA-binding protein blot assay and in vitro binding studies, we identified lamin B1 as a MAR-binding protein, which provides evidence for a specific interaction of DNA with the nuclear lamina.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Probes / metabolism
  • DNA-Binding Proteins / metabolism
  • Lamin Type B*
  • Lamins
  • Nuclear Matrix / metabolism*
  • Nuclear Matrix / ultrastructure
  • Nuclear Proteins / metabolism*
  • Rats

Substances

  • DNA Probes
  • DNA-Binding Proteins
  • Lamin Type B
  • Lamins
  • Nuclear Proteins
  • lamin B1