Peritoneal and splenic B-1 cells are separable by phenotypic, functional, and transcriptomic characteristics

Eur J Immunol. 2004 Aug;34(8):2158-67. doi: 10.1002/eji.200424819.


B-1 cells constitute a distinct B cell population with unique phenotypic and functional characteristics. Although the origin of B-1 cells remains controversial, B-1 cells in different locations are generally considered to be part of the same pool. To determine the validity of this assumption, we examined peritoneal and splenic B-1 cells isolated by flow cytometric cell sorting from normal mice for several features. We found that splenic B-1 cells differ from peritoneal B-1 cells in terms of surface antigen expression, viability ex vivo, immunoglobulin secretion in vitro, stimulated cell cycle progression, and expression of Notch family, Notch-dependent, and Notch-associated genes. These results indicate that splenic and peritoneal B-1 cells are not the same and thus dispute the notion that B-1 cells are uniform, and may suggest that different subpopulations of B-1 cells arise separately, home individually, and/or are heavily influenced by local environmental factors.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocyte Subsets / classification
  • B-Lymphocyte Subsets / immunology
  • B-Lymphocyte Subsets / physiology*
  • B7-1 Antigen / immunology
  • Flow Cytometry
  • Gene Expression / physiology*
  • Gene Expression Profiling
  • Immunoglobulin M / immunology
  • Male
  • Mice
  • Peritoneum / immunology
  • Peritoneum / physiology
  • Spleen / immunology
  • Spleen / physiology


  • B7-1 Antigen
  • Immunoglobulin M