Expression of cyclooxygenase-2 in human esophageal squamous cell carcinomas

World J Gastroenterol. 2004 Aug 1;10(15):2168-73. doi: 10.3748/wjg.v10.i15.2168.


Aim: To determine whether cyclooxygenase-2 (COX-2) was expressed in human esophageal squamous cell carcinoma.

Methods: Quantitative reverse transcription-polymerase chain reaction (RT-PCR), western blotting, immunohistoc-hemistry and immunofluorescence were used to assess the expression level of COX-2 in esophageal tissue.

Results: COX-2 mRNA levels were increased by >80-fold in esophageal squamous cell carcinoma when compared to adjacent noncancerous tissue. COX-2 protein was present in 21 of 30 cases of esophageal squamous cell carcinoma tissues, but was undetectable in noncancerous tissue. Immunohistochemistry was performed to directly show expression of COX-2 in tumor tissue.

Conclusion: These results suggest that COX-2 may be an important factor for esophageal cancer and inhibition of COX-2 may be helpful for prevention and possibly treatment of this cancer.

MeSH terms

  • Adult
  • Aged
  • Blotting, Western
  • Carcinoma, Squamous Cell / metabolism*
  • Cyclooxygenase 2
  • Esophageal Neoplasms / metabolism*
  • Fluorescent Antibody Technique
  • Humans
  • Immunohistochemistry
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Membrane Proteins
  • Middle Aged
  • Prostaglandin-Endoperoxide Synthases / genetics
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction


  • Isoenzymes
  • Membrane Proteins
  • RNA, Messenger
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases