Evaluation of Newer Diagnostic Methods for the Detection and Differentiation of Entamoeba Histolytica in an Endemic Area

Trans R Soc Trop Med Hyg. Jul-Aug 2003;97(4):396-7. doi: 10.1016/s0035-9203(03)90068-x.


We evaluated the colorimetric polymerase chain reaction (PCR)-based method for the detection and differentiation of Entamoeba spp. and compared the efficacy of E. histolytica-specific antigen detection in faeces with the detection of specific antibodies to E. histolytica-specific antigen in faeces, by enzyme-linked immunosorbent assay. Faecal samples were obtained from patients attending hospital in Chandigarh, India, from March 2001 to February 2002. The PCR-based colorimetric method was found to be the most sensitive (100%) and it could differentiate between pathogenic and non-pathogenic Entamoeba spp. The present study also emphasized that the antigen detection system may prove to be a better diagnostic tool than the antibody detection system in endemic areas.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Animals
  • Antigens, Protozoan / analysis
  • Endemic Diseases
  • Entamoeba histolytica / classification
  • Entamoeba histolytica / immunology
  • Entamoeba histolytica / isolation & purification*
  • Entamoebiasis / diagnosis*
  • Entamoebiasis / epidemiology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Feces / parasitology
  • Humans
  • India / epidemiology
  • Parasitology / methods
  • Polymerase Chain Reaction / methods


  • Antigens, Protozoan