The Sema domain of Met is necessary for receptor dimerization and activation

Cancer Cell. 2004 Jul;6(1):75-84. doi: 10.1016/j.ccr.2004.06.013.


Hepatocyte growth factor (HGF) binds the extracellular domain and activates the Met receptor to induce mitogenesis, morphogenesis, and motility. The extracellular domain of Met is comprised of Sema, PSI, and four IPT subdomains. We investigated the contribution of these subdomains to Met receptor dimerization. Our observations indicate that the Sema domain is necessary for dimerization in addition to HGF binding. Treatment of Met-overexpressing tumor cells with recombinant Sema in the presence or absence of HGF results in decreased Met-mediated signal transduction, cell motility, and migration, behaving in a manner similar to an antagonistic anti-Met Fab. These data suggest that the Sema domain of Met may not only represent a novel anticancer therapeutic target but also acts as a biotherapeutic itself.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / metabolism
  • Antibodies, Monoclonal / pharmacology
  • Cell Movement*
  • Cross-Linking Reagents
  • Dimerization
  • Extracellular Matrix / metabolism*
  • Female
  • Hepatocyte Growth Factor / genetics
  • Hepatocyte Growth Factor / metabolism*
  • Humans
  • Mice
  • Mice, Nude
  • Precipitin Tests
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins c-met / antagonists & inhibitors*
  • Proto-Oncogene Proteins c-met / immunology
  • Proto-Oncogene Proteins c-met / metabolism*
  • Sequence Deletion
  • Signal Transduction*
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Cross-Linking Reagents
  • Hepatocyte Growth Factor
  • Proto-Oncogene Proteins c-met