Yeast display of antibody fragments: a discovery and characterization platform

J Immunol Methods. 2004 Jul;290(1-2):69-80. doi: 10.1016/j.jim.2004.04.009.


Yeast display of antibody fragments has proven to be an efficient and productive means for directed evolution of single-chain Fv (scFv) antibodies for increased affinity and thermal stability and, more recently, for the display and screening of a non-immune scFv and immune Fab libraries. A major strength of yeast display as a novel antibody discovery platform is the ability to characterize the binding properties, i.e., the affinity and epitope binding characteristics, of a clone without the need for subcloning, expression and purification of the scFv. This review focuses on novel attributes of yeast display for antibody engineering endeavors.

Publication types

  • Review

MeSH terms

  • Animals
  • Directed Molecular Evolution / methods*
  • Epitopes / genetics
  • Epitopes / immunology
  • Flow Cytometry
  • Humans
  • Immunoglobulin Fragments / genetics*
  • Immunoglobulin Fragments / immunology
  • Peptide Library
  • Precipitin Tests
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / immunology


  • Epitopes
  • Immunoglobulin Fragments
  • Peptide Library