IMGT/JunctionAnalysis: the first tool for the analysis of the immunoglobulin and T cell receptor complex V-J and V-D-J JUNCTIONs

Bioinformatics. 2004 Aug 4;20 Suppl 1:i379-85. doi: 10.1093/bioinformatics/bth945.

Abstract

Motivation: To create the enormous diversity of 10(12) immunoglobulins (IG) and T cell receptors (TR) per individual, very complex mechanisms occur at the DNA level: the combinatorial diversity results from the junction of the variable (V), diversity (D) and joining (J) genes; the N-diversity represents the addition at random of nucleotides not encoded in the genome; and somatic hypermutations occur in IG rearranged sequences. The accurate annotation of the junction between V, D, J genes in rearranged IG and TR sequences represents therefore a huge challenge by its uniqueness and complexity. We developed IMGT/JunctionAnalysis to analyse automatically in detail the IG and TR junctions, according to the IMGT Scientific chart rules, based on the IMGT-ONTOLOGY concepts.

Results: IMGT/JunctionAnalysis is the first tool for the detailed analysis of the IG and TR complex V-J and V-D-J JUNCTION(s). It delimits, at the nucleotide level, the genes resulting from the combinatorial diversity. It identifies accurately the D genes in the junctions of IG heavy (IGH), TR beta (TRB) and delta (TRD) chains. It delimits the palindromic P-REGION(s) and the N-REGION(s) resulting from the N-diversity. It evaluates the number of somatic hypermutations for each gene, within the JUNCTION. IMGT/JunctionAnalysis is capable of analysing, in a single run, an unlimited number of junctions from the same species (currently human or mouse) and from the same locus.

Availability: IMGT/JunctionAnalysis is available from the IMGT Home page at http://imgt.cines.fr

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Base Sequence
  • Gene Rearrangement, T-Lymphocyte*
  • Genes, Immunoglobulin
  • Genetic Variation / genetics*
  • Immunoglobulin Joining Region / genetics*
  • Immunoglobulins / genetics*
  • Molecular Sequence Data
  • Receptors, Antigen, T-Cell / genetics*
  • Sequence Analysis, DNA / methods
  • Software*

Substances

  • Immunoglobulin Joining Region
  • Immunoglobulins
  • Receptors, Antigen, T-Cell