Pim-1 expression in prostatic intraepithelial neoplasia and human prostate cancer

Prostate. 2004 Sep 1;60(4):367-71. doi: 10.1002/pros.20064.

Abstract

Background: PIM-1, an oncogene product of serine/threonine kinase, has been found to play an important role in induction/suppression of apoptosis, cell cycle progression, and transcriptional regulation by phosphorylating the target proteins involved in these processes. Recently, the expression of PIM-1 has been shown to correlate significantly with measures of prostate cancer clinical outcome.

Methods: Immunohistochemical analysis was used to characterize the patterns of PIM-1 expression in high grade prostatic intraepithelial neoplasia (HGPIN) and cancer in 121 radical prostatectomy specimens.

Results: Moderate to strong cytoplasmic staining was observed in 68% of cancers, 12% presented nuclear positivity as well. Moderate to strong expression was seen in 76% of tumors with Gleason score (GS) 7 or higher compared to 58% in tumors with GS 6 or lower (P = 0.04). The staining intensity was moderate or strong in 97% of HGPIN lesions. PIM-1 was overexpressed in HGPIN compared to cancer in 65% of cases. Expression in benign glands was negative or only weakly positive.

Conclusion: Our data suggest that PIM-1 overexpression in HGPIN may be an early event in the development of prostate malignancy. Additionally, PIM-1 expression provides supplementary information for distinguishing HGPIN from benign epithelium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Cell Transformation, Neoplastic
  • Diagnosis, Differential
  • Gene Expression Profiling*
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Prostatectomy
  • Prostatic Intraepithelial Neoplasia / diagnosis
  • Prostatic Intraepithelial Neoplasia / genetics*
  • Prostatic Intraepithelial Neoplasia / pathology*
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology*
  • Protein-Serine-Threonine Kinases / biosynthesis*
  • Proto-Oncogene Proteins / biosynthesis*
  • Proto-Oncogene Proteins c-pim-1

Substances

  • Proto-Oncogene Proteins
  • PIM1 protein, human
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-pim-1