The aim of this study was to investigate the relationship between the depth of the curve of Spee and positions of upper and lower incisors, overjet, overbite, and anterior lower crowding. The material consisted of lateral head films and dental casts of 137 untreated adolescent subjects, 76 girls and 61 boys, aged 13 to 16 years. The subjects were divided into three groups with normal Spee, flat Spee, and deep Spee and were compared with one another. Differences between the Spee groups and between sexes were assessed by means of analysis of variance and a post hoc multiple comparison test. In addition, correlation coefficients between the depth of curve of Spee and other variables were calculated. Finally, cephalometric measurements for all subjects were subjected to a multiple regression analysis, with the depth of curve of Spee as the dependent variable. The results showed that there were no statistically significant differences in the positions of upper and lower incisors and anterior lower crowding among the Spee groups. However, overjet and overbite demonstrated significant differences among the groups. Statistically significant correlations were found between the depth of curve of Spee and overjet and overbite. Multiple linear regression analysis revealed that chronological age and all other variables used in the study could account for only 28.7% of the total variance of the curve of Spee. The overbite alone explained 17.3% of the total variance of the curvature.