Lco1 is a novel widely expressed lamin-binding protein in the nuclear interior

Exp Cell Res. 2004 Aug 15;298(2):499-511. doi: 10.1016/j.yexcr.2004.04.028.


A-type lamins are localized at the nuclear envelope and in the nucleoplasm, and are implicated in human diseases called laminopathies. In a yeast two-hybrid screen with lamin C, we identified a novel widely expressed 171-kDa protein that we named Lamin companion 1 (Lco1). Three independent biochemical assays showed direct binding of Lco1 to the C-terminal tail of A-type lamins with an affinity of 700 nM. Lco1 also bound the lamin B1 tail with lower affinity (2 microM). Ectopic Lco1 was found primarily in the nucleoplasm and colocalized with endogenous intranuclear A-type lamins in HeLa cells. Overexpression of prelamin A caused redistribution of ectopic Lco1 to the nuclear rim together with ectopic lamin A, confirming association of Lco1 with lamin A in vivo. Whereas the major C-terminal lamin-binding fragment of Lco1 was cytoplasmic, the N-terminal Lco1 fragment localized in the nucleoplasm upon expression in cells. Furthermore, full-length Lco1 was nuclear in cells lacking A-type lamins, showing that A-type lamins are not required for nuclear targeting of Lco1. We conclude that Lco1 is a novel intranuclear lamin-binding protein. We hypothesize that Lco1 is involved in organizing the internal lamin network and potentially relevant as a laminopathy disease gene or modifier.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / genetics
  • Amino Acid Sequence / genetics
  • Base Sequence / genetics
  • Binding Sites / genetics
  • Carrier Proteins / genetics
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism*
  • Cell Nucleus / metabolism*
  • Cell Nucleus / ultrastructure
  • Cytoplasm / genetics
  • Cytoplasm / metabolism
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • HeLa Cells
  • Humans
  • Lamin Type A / metabolism
  • Lamin Type B / metabolism
  • Lamins / metabolism*
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Proteins / genetics
  • Nuclear Proteins / isolation & purification
  • Nuclear Proteins / metabolism*
  • Protein Binding / genetics
  • Protein Structure, Tertiary / genetics
  • Protein Transport / genetics


  • Carrier Proteins
  • DNA, Complementary
  • Lamin Type A
  • Lamin Type B
  • Lamins
  • Nuclear Proteins
  • lamin B1

Associated data

  • GENBANK/D50923