Papillary carcinoma of the thyroid: methylation is not involved in the regulation of MET expression

S Scarpino; A Di Napoli; M Rapazzotti-Onelli; E Pilozzi; L Ruco

doi:10.1038/sj.bjc.6601988

Papillary carcinoma of the thyroid: methylation is not involved in the regulation of MET expression

Br J Cancer. 2004 Aug 16;91(4):703-6. doi: 10.1038/sj.bjc.6601988.

Authors

S Scarpino¹, A Di Napoli, M Rapazzotti-Onelli, E Pilozzi, L Ruco

Affiliation

¹ Dipartimento di Diagnostica di Laboratorio e Patologia, II Facoltà di Medicina e Chirurgia, Ospedale Sant'Andrea, Via di Grottarossa 1035-1039, 00189 Università 'La Sapienza', Rome, Italy. stefania.scarpino@uniromal.it

Abstract

Hypomethylation has been reported to be responsible for the activation of several oncogenes. The possibility that hypomethylation is involved in the regulation of MET transcription was investigated through the analysis of the methylation status of one CpG island containing 43 CpGs in six cases of papillary carcinoma, in the corresponding normal thyroid tissue, and in two cases of hyperplastic goitre. Evidence of methylation was not found in any of the analysed CpG.

MeSH terms

Adult
Aged
Carcinoma, Papillary / genetics*
Carcinoma, Papillary / physiopathology
CpG Islands
DNA Methylation*
DNA, Neoplasm
Female
Gene Expression Regulation, Neoplastic*
Humans
Immunohistochemistry
Male
Middle Aged
Promoter Regions, Genetic
Protein Biosynthesis*
Proto-Oncogene Proteins / biosynthesis*
Proto-Oncogene Proteins c-met
Receptors, Growth Factor*
Thyroid Neoplasms / genetics*
Thyroid Neoplasms / physiopathology
Transcription, Genetic

Substances

DNA, Neoplasm
Proto-Oncogene Proteins
Receptors, Growth Factor
MET protein, human
Proto-Oncogene Proteins c-met