Improved cell growth and Taxol production of suspension-cultured Taxus chinensis var. mairei in alternating and direct current magnetic fields

Biotechnol Lett. 2004 Jun;26(11):875-8. doi: 10.1023/b:bile.0000025895.76394.ab.

Abstract

The growth of suspension cultures of Taxus chinensis var. mairei and Taxol production were promoted both by a sinusoidal alternating current magnetic field (50 Hz, 3.5 mT) and by a direct current magnetic field (3.5 mT). Taxol production increased rapidly from the 4th d with the direct current magnetic field but most slowly with the alternating current magnetic field. The maximal yield of Taxol was 490 microg l(-1) with the direct current magnetic field and 425 microg l(-1) with the alternating current magnetic field after 8 d of culture, which were, respectively, 1.4-fold and 1.2-fold of that without exposure to a magnetic field.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / radiation effects
  • Cell Culture Techniques / methods*
  • Cell Proliferation / radiation effects
  • Cell Survival / radiation effects
  • Cells, Cultured
  • Dose-Response Relationship, Radiation
  • Electromagnetic Fields*
  • Paclitaxel / biosynthesis*
  • Taxus / physiology*
  • Taxus / radiation effects*

Substances

  • Paclitaxel