Optimization of cryoprotectants for cryopreservation of rat hepatocyte

Biotechnol Lett. 2004 May;26(10):829-33. doi: 10.1023/b:bile.0000025886.57547.04.


Rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing either fetal bovine serum (FBS), glycerol, dimethyl sulfoxide (DMSO), sucrose or a mixture of these as a cryoprotectant. The best survival was with 10% (v/v) DMSO containing 30% (v/v) FBS using 5 x 10(5) hepatocytes ml(-1) at -70 degrees C for 5 d on type I collagen-coated dishes. After thawing, the cell viability was 81% determined by the MTT-test. The cryopreserved hepatocytes had the capacity of albumin synthesis similar to hepatocytes without cryopreservation. This result shows that cryopreservation of rat hepatocyte can be used for the evaluation of hepatic functions.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods
  • Cell Survival / drug effects
  • Cells, Cultured
  • Cryopreservation / methods*
  • Cryoprotective Agents / pharmacology*
  • Dimethyl Sulfoxide / pharmacology
  • Dose-Response Relationship, Drug
  • Glycerol / pharmacology
  • Hepatocytes / drug effects
  • Hepatocytes / physiology*
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Serum Albumin, Bovine / pharmacology


  • Cryoprotective Agents
  • Serum Albumin, Bovine
  • Glycerol
  • Dimethyl Sulfoxide